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(a) State four characteristics of enzymes (b) (i) List two digestive enzymes produced ...

(a) State four characteristics of enzymes
(b) (i) List two digestive enzymes produced in the duodenum of humans
(ii) Name the substrate that each enzyme listed in 2(b)(i) acts on.
(iii) State the products of each enzyme activity in 2(b)(ii)
(c) (i) State one way in which chlorophyll is important in plants
(ii) Name two macroelements that are required for the formation of chlorophyll in plants.
(iii) State one deficiency symptom of each macroelement named in 2 (c) (ii) above
(d) Outline the proce-dure used for testing for starch in a leaf
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    Correct Answer: Option n
    Explanation:
    (a). Characteristic of Enzymes;

    They are biological catalystsialter/speed up the rate of biological reactions
    remain chemically unchanged at the end of a reaction
    their actions are reversible
    they are specific in action/act on specific substrates
    enzymes are required in small amount
    are protein in nature
    they act best at optimum temperature/high temperature/denatures them/ low temperature inactivates them
    specific/sensitive to pH
    actions retarded by poisons/inhibitors
    some required co-enzymes to function.

    (b)i. Digestive enzymes produced in the duodenum of humans

    Amylase
    Trypsin/chymotrypsin
    Lipase

    ii. Substrates for each enzyme

    Amylase = starch
    Trypsin/chymotrypsin =protein
    Lipase = fats and oil

    iii. Product of enzyme activity

    Amylase = maltose
    Trypsin/chymotrypsin = peptones/polypeptides/peptide
    Lipase =glycerol and fatty acid

    (c)i. Importance of chlorophyll in plants
    Absorbs/traps sunlight; for photosynthesis
    ii. Macro-elements required for the formation of chlorophyll

    Nitrogen
    Magnesium
    Iron

    iii. Deficiency symptoms of each macro-element in (c)ii Nitrogen

    Chlorosis/yellowing of leaves
    Stunted growth
    Poor flower/fruit development

    MagnesiuM

    Poor growth
    Chlorosis/yellowing of leaves

    Iron

    Poor/stunted growth
    Chlorosis/yellowing of leaves

    (d) Procedure for test for starch

    Detach/pluck off/take leaf to be tested from plant after 4 to 6 hours of exposure to sun to enable it to form starch
    Put leaf in boiling water for about 30 seconds to kill the cells
    Put boiled leaf in 70% warm alcohol/ethylated spirit in water bath to decolorise the leaf Rinse decolorised/brittle leaf in running cold water to soften the leaf tissues/remove alcohol
    Spread out on white tiles/watch glass and add a few drops of iodine solution
    Allow to stand for a few minutes
    It turns blue-balck showing/indicating presence of starch.

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